Alpha Bright Serum

NOM ALPHA BRIGHT SERUM
Intensive spot correcting concentrate
INDICATIONS Brown spots induced by environmental (sun, drugs,
pollution) and hormonal changes (pregnancy, aging)
Lentigos, melasma
Post inflammatory pigmentation
KEY INGREDIENTS 8% stabilized L-ascorbic acid (vitamin C in its natural
form): antioxidant, limits melanine transfer, boosts epidermis
turnover (light exfoliation)
2% Phytic Acid: antioxidant, pollutant scavenger, regulates
pigmentation.
Acétyl Glycyl ß-Alanine: powerfull spot corrector and
lightening peptide, with 4-level mechanism on
melanogenesis.
EFG® solution (Essential Fraction of Ginkgo biloba)
Highly concentrated standardized extract containing
quercetines, gingkolides and bilobalides.
Perfume free, preservative free, paraben free, and
phenoxyethanol free, not tested on animals.
SKIN TYPE All skin types. Oil free texture adapted to normal and
combination skins. The Phytic Acid helps to normalize skin
sebum.
USE Apply morning and evening to the affected areas using a
gentle massage.
Wait a few seconds until the serum has penetrated before
applying the usual skincare and the sunscreen in case of
sun exposure.
Professional peeling: apply daily 2 weeks before to lower
skin pH, wait 24h after the peeling before use.
Laser and aesthetic treatment: wait 24h to 48h before use.
EUROPEAN RETAIL
PRICE
€ 62
PACKAGING
DESCRIPTION
Glass bottle + glass dropper
8 languages leaflet (FR/EN/NL/DE/IT/ES/PT/AR)
6 languages box (FR/EN/NL/DE/IT/ES)
LOGISTIC
INFORMATIONS
20ml
Code 110104, EAN 3770006425196
Box size: 115x36x64 ; unit weight : 0,085kg
MULTIPACKING 54 units per box
Multipacking box size: 600x240x146mm
Multipacking box weight: 4,98kgCONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
SCIENCE FROM NATURE
ALPHA BRIGHT SERUM is a high concentration and stable formula
with powerful natural active ingredients.
1 Powerful PROTECTIVE shield with antioxidant and antipollution action.
2 ANTI-SPOT and LIGHTNING intensive action

Reasons for skin pigmentation
The pigmentation processes
1. The melanin pigments (melanosomes) are produced at the basal layer
of the skin by the melanocytes.
2. They are transferred by the dendrites from the melanocytes to the
keratinocytes which are the constituents of the superficial layer of the
skin (epidermis).
3. During the epidermis turnover (30 days), the keratinocytes carry the
melanosome and move until the top until they die and disappear under
the form of dead skin.CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
The factors for pigmentation disorder:
The melanocyte activity is induced by the following factors:
• the α MSH hormone which activity is related by both UV and hormone
levels (changes with periods, menopause, aging)
• the inflammation and oxidative stress induced by pollution, acne and
cosmetic procedures such as laser, chemical peels, surgery.
• the excess of iron in the dermis caused by aging or injury. That is why
aging skin needs more vitamin C to control the iron levels in the skin
ACETYL GLYCYL ß-ALANINE: powerful potent spot corrector and
brightening peptide – How our whitening works?
ALPHA BRIGHT SERUM is the only product that will treat all the factors for
skin pigmentation with its complete formula:
1. It is a potent and stable antioxidant complex to block the effect of free
radicals (oxidative stress) induced by pollution, acne and cosmetic
procedures such as laser, chemical peel. In addition, the phytic acid has a
direct effect by neutralizing the pollutants.
2. Acetyl glycyl ß-alanine is a potent spot corrector and brightening peptide.
Acetyl glycyl ß-alanine, L-ascorbic acid (vitamin C) and phytic acid have a
direct action on the melanocyte and regulate its activity.
3. Vitamin C and phytic acid control iron levels in the skin.
4. Acetyl glycyl β-alanine and L-ascorbic acid limit melanin transfer by
weakening dendrite and causing phagocytosis of cells.
5. L-ascorbic acid and phytic acid allow the elimination of dead cells.
The activity of ALPHA BRIGHT serum on oxidative stress makes it the best
treatment to be used after aesthetic procedure to avoid the pigmentary
rebound, that is why the doctors recommend it to be used on a daily basis
after all pigmentation procedures.CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
Efficacy test on cell culture: effectiveness on 4 levels of melanogenesis
Level 1 – UV activation: Inhibits Stem Cell Factor (SCF) et Endothelin-1 (ET-1)
– Keratinocyte-derived factors
– Regulate melanocyte proliferation and differentiation
– Effective on melanocyte dendrite formation
Level 2 – Tyrosinas activation: downregulates MITF mRNA expression by 39%.CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
Level 3: Inhibits tyrosinase level up to 36.5% in protein level
Comparison with reference active ingredients (AA2G : Ascorbyl Acid2
Glucoside):
Inhibits melanin content up to 45.5%CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
Level 4: Inhibition of Melanosome Transfer – Phagocytosis
Microsphere-based phagocytosis assay: decrease of 55%CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
Ex-vivo Melanin Assessment: excellent ability on skin brightening:
Untreated tissue 2% Acétyl Glycyl ß-Alanine
In-vivo Age Spot Reducing Study, on 10 women for 2 months
Average spot reduction of 73%
Age spot reduction on 2 subjects:
Efficacy Test
Ex-vivo Melanin Assay (MelanoDermTM)
Day 9 Day 9
Untreated Tissue 2% GenoWhiteTM
2 % GenoWhiteTM achieves excellent ability on skin brightening.
by BioInnovation Laboratories, Inc. (USA)
Efficacy Test
In-vivo Age Spot Reducing Study
by AMA Laboratories, Inc. (USA)
Age Spot Reduction – 14, 28, 56 days
Day 0 Day 14
Day 28 Day 56
Averaged Age Spot Reduction (%)
-41.47
Efficacy Test
In-vivo Age Spot Reducing Study
Day 0 Day 14 Day 28 Day 56
Age Spot Reduction – 14, 28, 56 days
37.50% 75.54% 83.68%
Age Spot Reduction
(2% GenoWhiteTM application)
by AMA Laboratories, Inc. (USA)
Efficacy Test
In-vivo Age Spot Reducing Study
Age Spot Reduction – 14, 28, 56 days
Day 0 Day 14 Day 28 Day 56
32.05% 80.63% 87.40%
by AMA Laboratories, Inc. (USA)
Age Spot Reduction
(2% GenoWhiteTM application)
Efficacy Test
Ex-vivo Melanin Assay (MelanoDermTM)
Day 9 Day 9
Untreated Tissue 2% GenoWhiteTM
2 % GenoWhiteTM achieves excellent ability on skin brightening.
by BioInnovation Laboratories, Inc. (USA)CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
In-vivo full-face lightening study, on 20 women for 2 months
Increase in L* value associated with skin lightening by 36%
Efficacy Test
In-vivo Full Face Whitening Study
*Defined as the most natural, untanned skin
tone of each individual.
Skin Color Brightness/Lightness [L*]
[L
*]
58.09
0.00 %
58.88
13.16 %
59.49
23.34 %
60.24
35.79 %
64.11
100.00 %
55
56
57
58
59
60
61
62
63
64
65
Baseline Day 14 Day 28 Day 56 Clear Skin*
Result:
GenoWhiteTM demonstrated increase in L* value
associated with skin lightening.
The increase are considered statistically significant
at each evaluation time point. Efficacy Test
In-vivo Full Face Whitening Study
Day 0 Day 14 Day 28 Day 56
2% GenoWhiteTM cream topically applied on face
Efficacy Test
In-vivo Full Face Whitening Study
Day 0 Day 14 Day 28 Day 56
2% GenoWhiteTM cream topically applied on face CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
PHYTIC ACID: antioxidant, pollutant scavenger, regulates pigmentation.

Phytic acid is a unique active ingredient to prevent and
correct skin aging. Used in high concentration in our
serum, it is suitable for skin under oxydative stress,
notably in urban environment. In addition, is has an
excellent synergic effect with vitamin C.
Phytic acid is found in plant seeds. It serves as the main
storage form of phosphorus in the seeds. When seeds
sprout, phytic acid is degraded and the phophorus
released for use by the young plant.
Protection against pollutants
The latest dermatological clinical studies show that air pollution multiplies
undesirable UV radiation effects on skin cells- increasing the photo-actinic damages thereby causing wrinkles, brown spots, melasma and skin sagging.1
Phytic Acid has the strong ability to chelate multivalent metal ions, especially zinc,
calcium and iron. The binding can result in very insoluble salts that are poorly
absorbed. Alternatively, the ability of Phytic Acid to chelate minerals has been
reported to have some protective effects, such as decreasing iron-mediated cancer
risk and lowering serum cholesterol and triglycerides.
Powerful antioxidant
Phytic Acid is a powerful natural antioxidant2
It also decreases the rate of lipid peroxidation3. The lipid peroxidation is the
process in which free radicals “steal” electrons from the lipids in cell membranes by
a free radical chain reaction mechanism resulting in cell damages.
Moisturizing and sebum control
Use of a 0.5% phytic acid formula regulates the sebum level in both dry skins and
oily skins to a value between 100 and 130 μg/cm2, which is the value for a normal
skin. This result is due to absorption of phytic acid into the pores after its
penetration into stratum corneum and consequently maintaining the appropriate
amout of sebum secreted from the sebaceous gland.
1 Pollution as a Risk Factor for the Development of Melasma and Other Skin Disorders of
Facial Hyperpigmentation ‐ Is There a Case to Be Made? Journal of Drug in Dermatology.
April 2015. Wendy E. Roberts MD FAAD
2 Crit Rev Food Sci Nutr. 1995 Nov;35(6):495-508. Phytic acid in health and disease. Zhou
JR1, Erdman JW Jr.
3 Phytic Acid Protective Effect Against Beef Round Muscle Lipid Peroxidation. BEOM JUN
LEE, DELOY G. HENDRICKSCONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
Sebum value for dry skins and oily skins after applying a 0.5% phytic acid lotion for
7 weeks 4
Controls excess of melanine to prevent brown spot and lighten the skin
This result is due to the penetration of phytic acid into basale layer inhibiting the
activity of tyrosinase, involved in melanine synthesis.
Decreases the melanine value by 8% in 7 weeks, in vivo study with 20 volunteers5
Phytic Acid properties for skin
– Powerful antioxidant
– Chelates heavy metals and iron
– Moisturises and regulates sebum production
– Anti-cancer
– Regulates pigmentation
– No toxicity
4 Dr Zhong, Soongsil University
5 Dr Zhong, Soongsil UniversityCONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
VITAMIN C IN ITS NATURAL FORM (L-ASCORBIC ACID), STABILIZED AT 8%
In its natural form (L- ascorbic acid), Vitamin C is one of the most effective an- tiaging ingredients but also the most unstable:
– in surface, L-ascorbic acid boosts epidermis turnover to increase skin
radiance and refine skin texture.
– At dermal level numerous studies678 proved that L-ascorbic acid boosts
collagen synthesis to reduce wrinkles and smooth the skin.
– It is also one of the most powerful anti-free radicals able to protect from
oxidative factors responsible for the premature aging of the skin (UV, stress,
pollution.
– Blocks melanin transfer through a weakening action on dendrites9
The vitamin C is not synthetized by human body and needs external intake.

How do we stabilize L-Ascorbic Acid in high concentration?
Thanks to its control of the chemical characteristics of vitamin C, Alphascience has
developed a technology based on a unique associative complex of powerful
antioxidants, which ensures the stabilization of highly concentrated L-ascorbic
acid.
6 Nusgens BV, Humbert P, Rougier A, et al. Topically applied vitamin C enhances the mRNA
level of collagens I and III, their processing enzymes and tissue inhibitor of matrix
metalloproteinase 1 in the human dermis J Invest Dermatol 2001 ; 116 : 853-859
7 Stimulation of collagen gene expression by ascorbic acid in cultured human fibroblasts. A
role for lipid peroxidation? M Chojkier, K Houglum, J Solis-Herruzo and D A Brenner
8 Ascorbic acid enhances the expression of type 1 and type 4 collagen and SVCT2 in
cultured human skin fibroblasts. Y Kishimoto, N Saito, K Kurita, K Shimokado
9 Marta I. Rendon MD, Jorge I. Gaviria MD – Review of Skin-Lightening Agents –
Dermatologic SurgeryCONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
EFG®: Essential Fraction of Ginkgo biloba
It one of the more ancient tree species that
appeared 270 million year ago. Ginkgo
Biloba trees were also among the few living
things in the area to survive the blast of
Hiroshima. Later, scientific studies proved
their resistance to mutagenic agents.
Gingko biloba is used in traditional medicine
for its multiple properties: antioxidant, and
vasoprotective. It improves irrigation of
tissues and small blood vessels resistance.
We use a highly concentrated standardized extract containing quercetins,
ginkgolides and bilobalides.
– Powerful antioxidant
– ROS Scavenger
– Blocks peroxidases activity
– Blocks U.V activation
– Decreases melanin productions
– Blocks DOPA-Dopaquinone oxidation
– Improves irrigation of tissues
EFG will also improve irrigation of skin tissues to improve skin radiance, notably for
smokers and skins suffering from pollution.
CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
ALPHA BRIGHT SERUM – CLINICAL STUDIES
«6 MONTHS CLINICAL TRIAL TO ASSESS THE DEPIGMENTING EFFICIENCY
OF A STABLE ANTIOXIDANT SERUM VERSUS HYDROQUINONE 2%
WITH 40 PATIENTS ON FACIAL HYPERPIGMENTATION»
Dra Natalia Guadalupe Pérez Leal
Dra María Ivonne Arellano Mendoza
Hospital General de México Dr. Eduardo Liceaga, Mexico
ABSTRACT
This study is an evaluation of the depigmenting efficacy of an antioxidant serum
(ALPHA BRIGHT SERUM®) versus a reference treatment (Hydroquinone 2%) in 40
patients with melasma and post-inflammatory pigmentation for 6 months. This
clinical trial reveals both treatments used have similar efficacy. An average severity
decreases of 28% was observed in melasma and 41% severity decrease in postinflammatory hyper-pigmentation. The best responses were observed in melasma
patients, where some patients presented improvements of 71%.
RESULTS
• MELASMA
The overall MASI measurement of the whole face showed an average decrease of
28% after 6 months (p 0.204) with a MASI score decreasing from 19 to 14.
The comparison between the 2 treatments HYDROQUINONE 2% and ALPHA
BRIGHT SERUM® showed equal reduction of pigmentation after 2 months (-11% VS –
12%) and 4 months (-14% VS -12%).
The decrease continued between 4 months and 6 months by -14% when exclusively
treated with ALPHA BRIGHT SERUM®.
According to the graph below we can note the decrease of the MASI score by
HYDROQUINONE 2% treatment and ALPHA BRIGHT SERUM® at T0, T2 months, T4
months and T6 months.
• POST INFLAMMATORY HYPERPIGMENTATIONCONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
The measurement of PAHPI on both sides of the face showed a decrease of 41% (p
0.0001) after 6 months, decreasing from 13.2 to 7.7.
According to the graph below we can note the decrease of the PAHPI score by
HYDROQUINONE 2% treatment and ALPHA BRIGHT SERUM® at T0, T2 months, T4
months and T6 months.
CONCLUSION
Both treatments used for 6 months have similar efficacy on post-inflammatory
hyperpigmentation and melasma. An average decrease of 28% was observed in
melasma and 41% decrease in post-inflammatory pigmentation. One patient achieved
a decrease in pigmentation up to 71% on melasma.
The ALPHA BRIGHT SERUM® have antioxidant depigmenting action, also decreased
pigmentation after the use of hydroquinone 2% treatment. It can therefore be an
interesting option after hydroquinone treatment to manage residual pigmentation
and also as a maintenance therapy for melasma and PIH. Skin texture improved and
patients referred satisfaction with the treatment with this serum.
CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
ALPHA BRIGHT SERUM – TOLERANCY STUDIES

Performed by independent laboratories:
Cutaneous tolerance study with patch test on 12 subjects for 48 hours
– Mean Irritation Index (MII) = 0,14
– Alphascience ALPHA BRIGHT SERUM is considered as non-irritant.
Use test under medical control on 21 volunteers
– Excellente tolerance for 90% of volunteers
– 2 cases of dryness sensation after use, disappeared after applying a
moisturizing cream.
Evaluation of the ocular potential irritancy by the neutral red release assay.
– The study product presents not very important cytotoxicity.
Cosmetic product safety report by CERT
Analysis of the composition of cosmetic products, results of stability testing and
challenge testing :
– the product has no toxic effect found which could affect human health in normal or
reasonably foreseeable use
OBSERVATIONAL STUDY ON 14 PATIENTS
MAYUMI CLINIC TOKYO,
Patient #6 – Before Patient #6 – After 3 months: reduction
of brown spots and wrinkles

CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
CLINICAL EFFICIENCY OF
ALPHA BRIGHT SERUM
Monocentrical study in a dermatological clinic with 15 women, mean
age 55.
Application of ALPHA BRIGHT SERUM twice a day during 2 months.
Evaluation of spots evolutions by PSI Anti-Aging SD at T0, T28 et
T56. France, Spring 2016.
EXCELLENT SKIN TOLERANCE FOR 90% OF VOLUNTEERS
2 cases of dryness sensation after use, disappears after applying a moisturizer.
SPOTS INTENSITY
SPOTS SIZE
Case #1976
Case #3964
0
0
100%
100%
75%
75%
50%
50%
25%
25%
T0
T0
T0
T0
T28
T28
T56
T56
T56
T56
Intensity index Size index
100%
100%
86%
92%
78%
83%CONFIDENTIAL FOR INTERNAL USE – DO NOT DISTRIBUTE WITHOUT ALPHASCIENCE SAS AUTHORISATION
ALPHA BRIGHT SERUM – MAIN COMPETITORS

Product Picture Ref.
price
Active ingredients Alphascience
Added Value
ALPHASCIENCE
Alpha Bright
serum
62€
20ml
8% L-ascorbic acid
2% Phytic Acid
Acetyl Glycyl ß-Alanine
Ginkgo biloba concentrate
Perfume free, preservative
free, paraben free,
phenoxyethanol free
SKINCEUTICALS
Advanced
pigment
corrector
€106
30ml
0,3% salycilic acid: exfoliates
1% hydroxyphenoxy
propionic acid: limits
melanin transfer
0,5% ellagic acid
4% yeast extract: cutaneous
barreer
More powerful
active ingredient in
a better
combination
Powerful
antioxidants
Acétyl Glycyl ßAlanine
AURIGA
Melaclear serum
Melalight serum
€42
15ml
L-ascorbic acid
Phytic Acid
Phenoxyethanol,
Methylchloroisothiazolinone,
Methylisothiazolinone, Ethyl
Paraben, Butyl Paraben,
Isobutyl Paraben, Methyl
Paraben
Acétyl Glycyl ßAlanine : powerful
melanin regulator
Paraben free,
phenoxyethanol
free
DERMACEUTICS
Yellow Cream
€35
15ml
Glycolic acid
Salycilic acid
Arbutin
Kojic acid
Licorice
Low efficiency of
Arbutin, Kojic acid
Licorice